Download e-book for iPad: Advances in Microbial Physiology, Vol. 15 by A. H. , Tempest, D. W. , editors Rose

By A. H. , Tempest, D. W. , editors Rose

ISBN-10: 0080579752

ISBN-13: 9780080579757

ISBN-10: 0120277158

ISBN-13: 9780120277155

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I t is important to realize, therefbre. that even if’ biomass and growth-limiting substrate concentrations have reached steady states other culture parameters may still be exhibiting transient beha\ w u r . Pye (1969) made a very detailed investigation ofglycolvtic flux oscillations following the addition of glucose and trehalose to. yeast suspensions. Oscillations in NADH, concentration were thought to be induced by the allosteric activation of phosphofruktokinase. Such a control mechanism produced a predictable short-cycle oscillation and, due to the point stimulation (hexose addition), the oscillations eventually damped out.

Pye (1969) made a very detailed investigation ofglycolvtic flux oscillations following the addition of glucose and trehalose to. yeast suspensions. Oscillations in NADH, concentration were thought to be induced by the allosteric activation of phosphofruktokinase. Such a control mechanism produced a predictable short-cycle oscillation and, due to the point stimulation (hexose addition), the oscillations eventually damped out. More recently Poole et al. (1973) have attempted to resolve oscillatory respiratory activity in synchronously dividing populations of Schizosaccharomyces pombe.

J. TRlNCl than the rate of expansion of the colony, and hence do not affect the growth rate of the peripheral hyphae. Similarly the rate of diffusion of nutrients from uncolonized parts of the medium towards the margin colony is slow compared with the rate of expansion of most fungal colonies. The leading hyphae of fungal colonies are continually growing into uncolonized medium which has approximately the same composition and pH value as the uninoculated medium. However, in the case of bacterial colonies which expand at very slow rates (Pirt, 19671, there will be a tendency for secondary metabolites formed at the centre of the colony to diffuse through the medium and inhibit growth at the periphery of the colony.

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Advances in Microbial Physiology, Vol. 15 by A. H. , Tempest, D. W. , editors Rose


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