Advances in Botanical Research, Vol. 11 - download pdf or read online

By J.A. Callow, H.W. Woolhouse (Eds.)

ISBN-10: 0120059118

ISBN-13: 9780120059119

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This may be a more significant problem for biological specimens. Various more rapid processors for spectrum analysis are available (Oliver, 198 la). The method of estimating the intensity or photon-counting correlation function G ( 2 ) ( is ~ )illustrated in Fig. 9. The data shown in this figure have been simulated using the scheme described by Hughes et al. (1973). A light beam of constant average intensity is incident upon a detector. The instantaneous intensity is a C T c I - 10 n ( t )n 3 3 3 6 6 610 5 3 6 2 1 9 2 9 1 15 3 6 3 610 3 2 9 215 2 9 3 6 5 3 6 6 2 151 9 4 3 5 3 3 6 2 1 3 1 3 3 6 1 I 1 2 2 3 1 1 1 5 2 2101 3 L 5 2 i 6 z m z 3 4 3 1 1 0 1 6 2 6 4 2 6 2 5 1 9 1 5~.

Such a consistent pattern would be expected from a system such as the pollen tube, where the relative numbers and sizes of components remain fairly constant. It can, of course, be argued that the allocation of r values to these cellular components is arbitrary. Apart from 42 M. W. STEER ETAL. the increased signal levels found for the r2component from vesicle-rich areas mentioned earlier, this would mean that the local viscosities would have to be considerably higher, 75-750 CP for secretory vesicles and 25-500 CP for ribosomes (if these are assumed to be represented by the r2component).

11) is placed above the epiillumination system and the plane of polarization of the laser beam (initially vertically oriented) is rotated [using the half-wave (hi2) plate] until the undesired reflections are suppressed, when the plane of polarization is perpendicular to the analyzer. A quarter-wave plate (X/4) is then placed between the objective and the specimen and rotated until the optical axis is at 45" to the plane of polarization of the incident light. In this position plane-polarized light passing down through the h/4 plate, being back scattered or reflected in the specimen, reemerges through the X / 4 plate linearly polarized in a direction at right angles to the incident beam.

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Advances in Botanical Research, Vol. 11 by J.A. Callow, H.W. Woolhouse (Eds.)


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